Part:BBa_I13500:Experience

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User Reviews

UNIQ063c7b8e1b9aee79-partinfo-00000000-QINU

Antiquity

This review comes from the old result system and indicates that this part did not work in some test.

••••• iGEM Michigan 2013

The 2013 Michigan igem team successfully used this part to produce GFP. Fig 2. - An inducible fim transcriptor system changes states and produces protein output NEB 10-beta E. coli, which lack the native fim switch (fimS) and known fim recombinases, were co-transformed with K1077007(amilCP j23100 fim switch) or K1077003(GFP j23100 fim switch), and K1077002 (aTc inducible fimE, HSL inducible hbiF) and plated on to LB plates with or without inducer. A) Close up of 3 colonies on a plate containing K1077007 and K1077002 co transformants. Three distinct phenotypes were observed: Solid Blue colonies(bottom left), Mixed colonies that had distinct white and blue regions(bottom middle), and Solid White colonies(top right). Therefore, the uninduced fim transcriptor in the context of K1077002 is subject to leaky fimE and/or hbiF activity. B) Quantification of the phenotypes observed on the plate in A. C) When induced with 4.32µM aTc and grown overnight (left) , no GFP is produced as expected due to induced fimE turning the switch OFF. When induced with 1µM HSL and grown overnight (right), GFP is produced as expected due to induced hbiF turning the switch ON.

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UNIQ063c7b8e1b9aee79-partinfo-00000003-QINU